Device

Part:BBa_J119397:Design

Designed by: Andrew Hunn   Group: Eckdahl Lab   (2016-07-06)


pJC173b gIII neg (Carlson et al. 2014)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
    Illegal AgeI site found at 2661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4501
    Illegal SapI site found at 3180


Design Notes

No BbsI sites in J100265 plasmid.


Source

iPCR of J100265 plasmid with primers that introduce BbsI site for Golden Gate Assembly.

References